Description
The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human SARS-CoV-2 (Covid-19) spike proteins present in the sample are bound by the capture antibodies. After incubation the wells are washed and followed by HRP- conjugated Detection Antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human SARS-CoV-2 (Covid-19) spike proteins in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm